Environmental Engineering Process Laboratories (Cook Campus)
These laboratories
are equipped to establish, monitor, and analyze
physical, chemical, and biological processes in engineered and natural systems. A
Gas Chromatography (GC) system equipped with Flame Ionization and Electron Capture
detection is used for quantifying volatile compounds in gas samples. There are two
high-performance liquid chromatography (HPLC) systems used for quantifying chemical
pollutants or microbial metabolites. An Ion Chromatography (IC) system is
available for quantifying dissolved charged chemical species in natural and
treated water. An Inductively Coupled Plasma-Optical Emission Spectrometry
(ICP-OES) system is available for the quantitation of trace elements in soils
or water. A supercritical CO2 extractor is used for activated
carbon regeneration and extraction of organic pollutants from soil and sediment
matrices. The laboratory is equipped for analyses of microbial communities via:
phase-contrast microscopy for enumeration and identification; spread or
membrane plating for selective cultivation; and DNA extraction, visualization,
polymerase chain reaction (PCR) amplification, and quantitative PCR for
detection and identification. A UV-visible spectrophotometer, various ovens,
dissolved oxygen meters, pH meters, and electronic balances are also available.
Physical
and chemical processes are demonstrated using a computer-controlled
flow-through reactor system with multiple sensors. The system allows students
to establish and monitor processes in a plug flow reactor (PFR), a completely
mixed flow reactor (CMFR), CMFRs in series, and other reactor configurations used
in water and wastewater treatment. Jar test systems are available for investigation
of interparticle interactions resulting in turbidity removal. Bench scale
batch reactors are employed for investigation of chemical reactions, ion
exchange, and adsorption processes. Computer software packages for activated
carbon adsorber design, air stripper design, and modeling of pollutant
distributions among multimedia are available.
Biological
processes are investigated using small-scale reactors. Students establish anaerobic digesters that are
monitored for COD removal, fatty acid production, and methane production.
Nonlinear curve-fitting is used to parameterize a biokinetic model of the
digestion process. Bioremediation techniques are tested in microcosms using
soils collected by students. Removal of gasoline and chlorinated solvents are
evaluated by monitoring contaminant profiles and microbial populations. In situ
water quality is measured in the field using handheld meters equipped with
probes for dissolved oxygen, pH, conductivity, and turbidity. Biochemical oxygen demand (BOD), chemical
oxygen demand (COD), bacterial indicator plate counts, and nutrient (phosphorus
and nitrogen) analysis are carried out using standard methods or commercial
(Hach) kits.
Environmental Engineering Laboratory (Busch Campus)
This laboratory is equipped for performing the
analysis of water and wastes and the unit processes associated with treatment.
Molecular level and advanced analytical chemical measurements are available for
complex environmental samples, including water and air matrices. The microbiological
equipment includes a cell homogenizer for DNA extraction, quantitative PCR
(qPCR), PCR, fluorescent microscope, microcentrifuge, electrophoresis
equipment, gel imager, constant-temperature water baths, incubators, and
laminar flow hoods. Analytical chemistry equipment includes an FTIR microscope, high-pressure
liquid chromatograph (HPLC) with UV detection, a total carbon/total nitrogen
analyzer, pH meters, field meters for conductivity/pH/ORP, chemical fume hood,
drying and annealing ovens, centrifuge, mixing devices, chemical metering
pumps, spectrophotometers (including Hach), and turbimeters. A variety of field
sampling equipment, devices to simulate unit processes (e.g., jar test apparatus,
annular biofilm reactor, sunlight simulator), and refrigerated storage is available.
Air Sampling and Analysis Laboratory (Cook Campus)
This laboratory is equipped to provide
students with experience in ambient and indoor air sampling. Students learn how
to use air flow rate and velocity measurement devices (i.e., wet test meter,
dry gas meter, bubble calibrator, mass flow meter, rotameters, S-type and
regular pitot tubes), particle samplers (i.e., Button inhalable aerosol
sampler, IOM inhalable aerosol sampler, PM2.5 impactor, cyclone), particle
measurement instruments (i.e., optical particle counter, condensation particle
counter, aerosol mass photometer), and bioaerosol samplers (i.e., 1-stage
Anderson impactor, 6-stage Anderson impactor, and portable bioaerosol samplers).
Students gain experience in analyzing gases using Draeger tubes and ozone
meters.
Soil and Water Laboratory (Cook Campus)
This laboratory is equipped to teach students how to determine the
properties of soils. Ovens and electronic balances are available for soil moisture
content analysis. pH meters are available for characterization of soil and
water samples.
Instruments for manual
or automated measurement of soil particle size distributions; and sensor-enabled
measurement of soil water content and pressure potential are available. Pressure
extractors and automatic systems to measure water retention curves and
saturated hydraulic conductivity, along with two types of tension
infiltrometers that can be connected to data loggers for automatically
quantifying water movement through unsaturated soils and sediments enable study
of flow in porous media. A physical aquifer
model allows students to visualize and manipulate movement of water and
contaminants in a groundwater environment.